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次级电子和背散射电子可以用于成像，兩者用處不一，前者多用在顯示物體表面起伏，後者則是用在顯示物體原子序的差異。 表面分析[编辑]
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最常见的扫描电子显微镜模式是检测由电子束激发的原子发射的二次电子（secondary electron）。可以检测的二次电子的数量，取决于样品测绘学形貌，以及其他因素。通过扫描样品并使用特殊检测器收集被发射的二次电子，创建了显示表面的形貌的图像。它还可能产生样品表面的高分辨率图像，且图像呈三维，鉴定样品的表面结构。
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On the other hand, microbial specimens need to have fixation, dehydration, and drying to be able to maintain the structural functions in the cells and to avoid collapsing of the cells when exposed to the superior vacuum with the microscope.